PCR Equipment and Supplies
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Standard Buffer both detergent free, 1,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq are Triton/Tween free 10x Ammonium Buffer and 10x Standard Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul , 5,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. No reaction buffers included.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Combination Buffer, Tween free, 2,500U
AS ONE HS Taq DNA Polymerase is a hot start polymerase designed to diminish the formation of non-specific priming events during reaction set up and the first ramp of thermal cycling. HS Taq features high specificity and sensitivity, greater yield compared to standard DNA polymerases. HS Taq enables the detection of low abundance tragets. HS Taq Polymerase includes Tween free 10x Combination Buffer.
Boca Scientific Inc SD Polymerase, 10 U/ul, 200 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium and detergent free, 5,000U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes Tween/Triton/magnesium free 10x Ammonium Buffer and 10x Combination Buffer
Meridian Life Science Gly-Free DNA Pol I Klenow exo-, 50UL
Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is a high concentration enzyme (50 U/uL) has mutations (D355A, E357A) which eliminates the 3’-5’ exonuclease activity. It is designed for dA and dT tailing for adapter ligation in next generation sequencing library preparation. The enzyme displays moderate strand displacement activity and can also be used to generate probes using random primers, dideoxy sequencing and random priming labelling. Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready Klenow Reaction Buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Standard Buffer both Magnesium and detergent free, 2,500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes Tween/Triton/magnesium free 10x Ammonium Buffer and 10x Standard Buffer.
Bioss RNA polymerase beta Recombinan
RNA polymerase beta Primary Antibody Unconjugated Host Species Rabbit Target Species E coli Clonality Monoclonal Applications WB IP RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, 500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq with Red Dye DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Ammonium Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Magnesium free and Tween free, 2,500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes Tween/magnesium free 10x Ammonium Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium free, 2,500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc High Fidelity Taq Polymerase, 1,000U
AS ONE PR Polymerase is a thermostable enzyme with PR ability, which can be used in primer extension reactions and other molecular biology applications. PR Polymerase exhibits both 5 prime to 3 prime DNA polymerase activity and 3 prime to 5 prime PR exonuclease activity. It is recommended for applications, which require extremely high fidelity or blunt ending. Optimal reaction conditions are achieved by using the 10x Ammonium buffer containing MgCl2 provided with the enzyme. 25 mM MgCl2 is also included separately, in case a higher MgCl2 concentration is required for a specific reaction.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Combination Buffer, Tween free, 500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Tween free 10x Combination Buffer.
As One International Inc Red-Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 10,000Rxn
AS ONE Taq DNA Polymerase with Red Dye Master Mix is a ready-to-use 1.1x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. An inert red dye and a stabilizer are also present to allow direct loading of the final products onto a gel for analysis. AS ONE Taq DNA Polymerase with Red Dye Mix offers several advantages. Set up time is significantly reduced. There is no need to buy and use separate loading dyes to load reaction products onto agarose gels for electrophoresis and subsequent visualization. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.